Journal: iScience

Article Title: Bizonal cardiac engineered tissues with differential maturation features in a mid-throughput multimodal bioreactor

doi: 10.1016/j.isci.2022.104297

Figure Lengend Snippet: Bioreactor design (A) Representation of the modular bioreactor incorporated with 24-well culture plates. The 3D printed bioreactor frame (c.) is placed between the 24-well plate (d.) and the lid (a.). It is possible to combine 6 independent mechanical stimulation modules (b.), allowing independent mechanical stimulation of 4 separate culture well rows (B and C). (D) Representation of ECTs cast in between 2 metal wires of the mechanical stimulation module. (E) Images of the cell-based fibrin gel generation in custom-made silicon mold. (F) Bioreactor’s modules functioning scheme: flexible wires provide resistance during auxotonic contraction and serve as sensors for measuring the contractile force F (a.); rigid wires are fixed in a moving shaft, connected to a driving stepper motor for delivery of active cyclical stretching (b.); the wire couple in between which the ECT is generated are used as conductive electrodes and connected to an electrical stimulator for application of electrical stimuli (c.).

Article Snippet: The bioreactor consisted of a 3D printed frame ( A-c.), made of polylactide acid (PLA) (MakerBot Replicator + printer, USA), and the mechanical stimulation module ( A-b.) positioned in a conventional 24-well culture plate ( A-d.).

Techniques: Generated